Zhijian Liu,Xiangying Kong,Yanping Long,Sirui Liu,Hong Zhang,Jinbu Jia,Wenhui Cui,Zunmian Zhang,Xianwei Song,Lijuan Qiu,Jixian Zhai,Zhe Yan.

Nature Plants,2023,IF 18.00

DOI:10.1038/s41477-023-01387-z

ABSTRACT:

Legumes form symbiosis with rhizobium leading to the development of nitrogen-fixing nodules. By integrating single-nucleus and spatial transcriptomics, we established a cell atlas of soybean nodules and roots. In central infected zones of nodules, we found that uninfected cells specialize into functionally distinct subgroups during nodule development, and revealed a transitional subtype of infected cells with enriched nodulation-related genes. Overall, our results provide a single-cell perspective for understanding rhizobium–legume symbiosis.