Lei Dekun; Jian Anqi; Huang Xianbo; Liu Xi; Chen Liangming; Bai Wenting; Cheng Siqi; He Xiaodong; Xiong Yehui; Yu Xiaowen; Wang Chaolong; Zheng Hai; You Shimin; Wang Qiming; Lu Jiayu; Hu Yang; Xie Zhenwei; Jiang Ling; Zhang Xin; Ren Yulong; Lei Cailin; Cheng Zhijun; Lin Qibing; Wu Chuanyin; Zhu Shanshan; Zhao Zhigang; Wan Jianmin. 

Plant Biotechnology Journal, 2023, IF 13.80

DOI:10.1111/pbi.14140

ABSTRACT:

In China, the Taigu-dominant male-sterile gene Ms2 has been widely used for population improvement by recurrent selection in wheat conventional breeding (Ni et al., 2017; Xia et al., 2017). However, such an approach has not been possible in rice. In this study, we have cloned the Dms1 locus and demonstrated why the male sterility is dominant in the Dms1. The use of dominant male sterility (Dms) lines for recurrent selection can at least save one generation of selfing, compared to recessive male sterility lines. Thus, isolation of Dms causal genes will facilitate creation of Dms in other varieties. Male sterility in Dms1 is a much-preferred line in rice breeding. The Dms1 line is an ideal line widely used in the recurrent selection breeding strategy. Identification of Dms1 provides opportunity to develop other dominant male sterility lines in various genetic backgrounds by transformation approach, which will increase efficiency and reduce cost in breeding programmes. Recent studies have discovered a number of genes involved in male gametogenesis, and their malfunctionization leads to male sterility. It is possible that the activated gametogenesis-specific expression of OsRIP1 directly or indirectly affects translation of some, if not all, of those genes, resulting in defective gametogenesis. Mammals suffer from toxicity when accidently foraging plants containing RIPs, and the uptaken RIPs in mammals specifically interact with stalk subunit of ribosome, then depurinate 28SrRNA (Grela et al., 2019). The damaged ribosome is no longer a functional organelle for normal protein translation. However, RIPs exist in plants, but cannot depurinate plant ribosomes and its toxicity to plants is unclear (Nielsen and Boston, 2001; De Zaeytijd and Van Damme, 2017). Our results also indicate that OsRIP1 inhibits translation function of ribosome not by 28SrRNA depurination. As the cytotoxicity of OsRIP1 is likely conserved in monocot and dicot plants, we anticipate OsRIP1 can be employed to create new Dms lines, for example, by anther-specific expression in other crops.