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Genome-wide proteomic profiling reveals the role of dominance protein expression in heterosis in immature maize ears

  作 者:Xiaojiao Hu, Hongwu Wang, Kun Li, Yujin Wu, Zhifang Liu & Changling Huang

  影响因子: 4.259

  刊物名称: Scientific reports

  出版年份: 2017

  卷: 7   期:23 November   页码: 16130


  文章摘要 :

  Heterosis refers to the phenomenon in which hybrid progeny show superior performance relative to their parents. Early maize ear development shows strong heterosis in ear architecture traits and greatly afects grain yield. To explore the underlying molecular mechanisms, genome-wide proteomics of immature ears of maize hybrid ZD909 and its parents were analyzed using tandem mass tag (TMT) technology. A total of 9,713 proteins were identifed in all three genotypes. Among them, 3,752 (38.6%) proteins were diferentially expressed between ZD909 and its parents. Multiple modes of protein action were discovered in the hybrid, while dominance expression patterns accounted for 63.6% of the total diferentially expressed proteins (DEPs). Protein pathway enrichment analysis revealed that high parent dominance proteins mainly participated in carbon metabolism and nitrogen assimilation processes. Our results suggested that the dominant expression of favorable alleles related to C/N metabolism in the hybrid may be essential for ZD909 ear growth and heterosis formation. Integrated analysis of proteomic and quantitative trait locus (QTL) data further support our DEP identifcation and provide useful information for the discovery of genes associated with ear development. Our study provides comprehensive insight into the molecular mechanisms underlying heterosis in immature maize ears from a proteomic perspective