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种质资源抗逆性评价 组长张辉 - 中国农业科学院作物科学研究所


 


姓  名: 张 辉      性 别: 男
职  称: 研究员   
联系电话:010-62186649      
电子邮箱:zhanghui06@caas.cn       
个人网页:
课 题 组:抗逆种质资源

组长简介:

张辉,三级研究员,博士生导师。1980.9-1984.7,天津南开大学本科学习,获遗传学理学学士学位;1998.10-2001.11,英国John Innes Centre从事博士学位研究,获分子生物学理学博士。1984.8-1987.8,1988.8-1996.7,1997.9-1998.9,2001.12-2003.6,中国农科院品资所,从事麦类作物种质资源的收集、农艺性状鉴定评价等;1987.9-1988.7,参加中央讲师团,赴黑龙江黑河市教师进修学校任数学老师;1996.8-1997.8,作为中欧访问学者,在英国John Innes Centre从事小麦比较基因组研究;2003.7- ,中国农科院作科所,任种质资源抗逆性评价创新小组组长,主要从事作物种质资源抗逆研究。
研究方向:  
抗逆种质资源收集、鉴定评价及抗逆机理研究
主要贡献:  
对我国沿海地区11个省市耐盐碱种质资源进行了系统的考察与收集,明确了该地区耐盐碱种质资源的分布状况及其演变规律。在比较基因组学研究中,明确了小伞山羊草U基因组、高大山羊草S基因组与小麦基因组之间的部分同源关系。利用转录组、蛋白质组学等方法,对小麦TaPPDK1基因、TaLEA1、TaLEA2基因、TaSAUR基因进行功能鉴定,证明这些基因的过表达可以显著提高转基因植物的抗盐性。在大豆小RNA及其靶基因的功能研究中,明确了大豆GmNFYA3 是NFY复合物的一个亚基,由miR169调控,受逆境胁迫诱导、呈组成型表达模式;大豆gma-miR394a的靶基因为Glyma08g11030,gma-miR394a能够提高转基因拟南芥的抗旱能力;大豆miR169靶基因GmNFYA3是NFY复合物的一个亚基,受逆境胁迫诱导表达,呈现组成型表达模式,GmNFYA3蛋白定位于细胞核,过表达GmNFYA3增强对干旱的忍耐能力,而对ABA和NaCl敏感。大豆抗逆基因编辑技术(CRISPR-Cas9)研究中,构建了大豆U6启动子驱动的pCas9-GmU6-sgRNA表达载体和拟南芥U6-26启动子驱动的pCas9-GmU6-sgRNA表达载体,在大豆原生质体和发状根中获得了基因的单、双突变。
主持、参加国家转基因重大专项、平台、保种、863、973、948、自然基金等项目约20余项;发表论文50余篇;先后获国家科技进步一等奖1项、团队奖1项,省部级科技进步一等奖1项、二等奖2项;培养博士生7名、硕士生12名。
在研科研项目:  
1. 转基因生物新品种培育重大专项
2. 科技基础平台服务项目
3. 保种项目
主要论文和著作:  
1. Qiyan Jiang, Xiaojuan Li, Fengjuan Niu, Xianjun Sun, Zheng Hu, Hui Zhang*. iTRAQ-based Quantitative Proteomic Analysis of Wheat Root in Response to Salt Stress. Proteomics, 2017, 17,8,1600265
2. Qiyan Jiang, Fengjuan Niu, Xianjun Sun, Zheng Hu, Xinhai Li, Youzhi Ma*, Hui Zhang*. RNA-seq analysis of unintended effects in transgenic wheat overexpressing the transcription factor GmDREB1. The Crop Journal, 2017, 5(3):207-218.
3. Qiyan Jiang, Xianjun Sun, Fengjuan Niu, Zheng Hu, Rui Chen*, Hui Zhang*. GmDREB1 Overexpression Affects the Expression of MicroRNA in GM Wheat Seeds. Plos One. 2017,12(5):e0175924
4. Yuan Guo, Qiyan Jiang*, Zheng Hu, Xianjun Sun, Shoujin Fan, Hui Zhang*. Function of the auxin-responsive gene TaSAUR75 under salt and drought stress. The Crop Journal, 2017, https://doi.org/10.1016/j.cj.2017.08.005
5. Li Ming, Hu Zheng, Jiang Qi-yan, Sun Xian-jun, Guo Yuan, Qi Jun-cang*, Zhang Hui*. GmNAC15 overexpression in hairy roots enhances salt tolerance in soybean. Journal of Integrative Agriculture, 2017, 16(0): 60345-7
6. Xianjun Sun, Zheng Hu, Rui Chen, Qiyang Jiang, Guohua Song, Hui Zhang* Targeted mutagenesis in soybean using the CRISPR-Cas9. Scientific Reports, 2015, 5:10342
7. Chen Rui*,Jiang Nan,Jiang Qiyan,Sun Xianjun,Wang Yong,Zhang Hui,Hu Zheng* Exploring microRNA-like small RNAs in the filamentous fungus Fusarium oxysporum. PLos One,2014, 9(8): e104956-e104956
8. Qiyan Jiang, Zheng Hu, Hui Zhang*, Youzhi Ma*. Overexpression of GmDREB1 improves salt tolerance intransgenic wheat and leaf protein response to high salinity. The crop journal, 2014,Z(1):120-131
9. Zheng Hu, Qiyan Jiang, Hui Zhang*. Prediction and identification of natural antisense transcripts and its small RNAs in soybean (Glycine max). BMC genomics, 2013, 14:280
10. Zheng Hu, Qiyan Jiang, Zhiyong Ni, Rui Chen, Shuo Xu, Hui Zhang*. Analyses of a Glycine max Degradome Library Identify microRNA Targets and MicroRNAs that Trigger Secondary SiRNA Biogenesis, Journal of Integrative Plant Biology, 2013, 2:160–176
11. Zhiyong Ni, Zheng Hu, Qiyan Jiang, Hui Zhang*. GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress. Plant Mol. Biol, 2013, 82:113–129
12. Qiyan Jiang, Zheng Hu, Xinglai Pan, Hui Zhang*. Comparative Proteomic analysis of wheat (Triticum aestivum L.) hybrid necrosis. Journal of Integrative Agriculture, 2013, 12, 101-108
13. Zhiyong Ni, Zheng Hu, Qiyan Jiang, Hui Zhang*. Overexpression of gma-MIR394a confers tolerance to drought in transgenic Arabidopsis thaliana. Biochemical and Biophysical Research Communications, 2012, 427, 330–335
14. Rui Chen, Zheng Hu and Hui Zhang*. Identification of MicroRNAs in Wild Soybean (Glycine soja). Journal of Integrative Plant Biology, 2009, 51(12): 1071-1079.


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